A simple, selective, rapid, precise and economical UV spectrophotometric method and reverse phase high performance liquid chromatography method has been developed for the simultaneous estimation of paracetamol and chlorxazone from pharmaceutical formulation. The primary method was based on UV-spectrophotometric determination of paracetamol and chlorzoxazone, using simultaneous equation method. Its absorbance measurement at 282.5 nm (λmax of chlorzoxazone) and 248.0 nm (λmax of paracetamol) in methanol and the linearity was obtained in the range of 5 – 25 μg/ml for paracetamol and chlorzoxazone. The secondary method was based on RP- HPLC separation of the paracetamol and chlorzoxazone in reverse phase mode. Linearity was obtained in the concentration range of 100-500 μg/ml for paracetamol and 50-250 for the chlorzoxazone. Both these methods have been successively applied to pharmaceutical formulation and were validated according to ICH guidelines. The developed methods are validated in terms of specificity, selectivity, accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability.
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